Identifying non-disease patients using a disease related assay and analysis in the liquid biopsy

An imaging structure identification system comprising:

• an optical imaging system configured to illuminate a liquid biopsy sample for a subject, the liquid biopsy sample having one or more biological structures that are labeled with one or more fluorophores associated with a fluorescence assay for a disease state allowing detection of emitted electromagnetic radiation from the liquid biopsy sample as image data;
• and
• a processing system configured to:
• generate images of the one or more biological structures for the subject from the image data, detect and determine a plurality of features from the images or the image data, and form biological structure identification buckets from the plurality of features, each biological structure identification bucket identifying imaging structures that are similar in type;
• generate a subject profile of biological structure identification buckets for rare imaging events for the subject;
• compare the subject profile with a first predetermined profile for subjects not having the disease state;
• and
• identifying the subject as being at reduced risk for having the disease state if the subject profile is similar to the first predetermined profile for subjects not being in the disease state, wherein if the subject profile is not similar to the first predetermined profile for subjects not being in the disease state it is not determinative if the subject is in the disease state.