Systems and Methods for Hyperspectral Microscopy

A microscopy system comprising:

• a laser configured to output a first beam and a second beam;
• a first optical system configured to receive the first beam, the first optical system comprising:
• a first pulse shaper configured to shape a first component of the first beam in at least one of a spectral aspect or a temporal aspect, the first pulse shaper including a first diffraction grating, a first achromatic half-wave plate, a first lens, and a first two-dimensional (2D) spatial light modulator (SLM);
• and
• a second optical system configured to receive the second beam.

A method of microscopy comprising:

• outputting a first laser beam to a first optical system and a second laser beam to a second optical system;
• by a first beam splitter of the first optical system, splitting the first beam into a first component and a second component;
• by a first photonic crystal fiber of the first optical system, modifying a bandwidth of the first component;
• by a first pulse shaper of the first optical system, shaping at least one of a spectral aspect or a temporal aspect of the first component;
• by a first beam combiner of the first optical system, combining the shaped first component with the second component to generate a first combined beam;
• by a second beam splitter of the second optical system, splitting the first beam into a third component and a fourth component;
• by a second photonic crystal fiber of the second optical system, modifying a bandwidth of the third component;
• by a second pulse shaper of the second optical system, shaping at least one of a spectral aspect or a temporal aspect of the third component;
• by a second beam combiner of the second optical system, combining the shaped third component with the fourth component to generate a second combined beam;
• and
• by a third beam combiner, combining the first combined beam with the second combined beam to generate an output beam.