Methods of diagnostic screening and early detection of adenomyosis

A method of treating a benign gynecologic condition in a patient in need thereof, the method comprising:

• a) diagnosing the benign gynecologic condition in the patient by:
• i) obtaining a biological sample from the patient;
• ii) measuring the levels of five or more biomarkers comprising protein biomarkers, metabolites biomarkers, or a combination thereof in the sample obtained in (i);
• and
• iii) diagnosing the patient with the benign gynecologic condition if the patient has levels of at least five or more biomarkers altered from a predetermined threshold;
• and
• b) administering a therapeutic amount of a treatment to the patient if the patient is diagnosed with a benign gynecologic condition.

A method of distinguishing between adenomyosis and endometriosis in a subject, the method comprising:

• a) obtaining a biological sample from the subject;
• and
• b) measuring the levels of five or more biomarkers comprising protein biomarkers, metabolites biomarkers, or a combination thereof in the sample obtained in (a);
• wherein the subject is determined to have adenomyosis if the levels of at least five or more biomarkers are altered from a predetermined threshold.