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US 2025/0147041 A1Published application
Amyloid beta detection by mass spectrometry
AssigneeQuest Diagnostics Investments Incorporated
InventorsDiana Tran, Darren Weber, Nigel Clarke
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Abstract
Methods for the detection or quantitation of amyloid beta include detecting amyloid beta or fragments thereof by mass spectrometry. The methods may also include determining the ratio of amyloid beta 42 (Aβ42) to amyloid beta 40 (Aβ40). Such methods may include the diagnosis or prognosis of Alzheimer's disease or dementia.
Claims19
A method for determining the amount of amyloid beta in a sample, said method comprising:
- (a) purifying amyloid beta in the sample, wherein the amyloid beta comprises Aβ40 having the sequence
- DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV (SEQ ID NO:1) or A62 42 having the sequence
- DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA (SEQ ID NO:3);
- (b) ionizing amyloid beta in the sample to produce a precursor ion of amyloid beta;
- (c) generating one or more fragment ions of amyloid beta;
- and
- (d) determining the amount of the ion(s) from step (c) or (d) or both by mass spectrometry;
- wherein the amount of the amyloid beta ion(s) is related to the amount of amyloid beta in the sample.
5 more elements
Dependencies are inferred from the claim text.