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AssigneeThe Regents of the University of Colorado, a body
InventorsAnatoli Pinchuk, Tetyana Sergeyeva, Daria Yarynka, Vladimir Chegel, Andri Lopatynskyi
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A highly sensitive technology enabling selective recognition of aflatoxins such as in contaminated cereal samples. Methods for the synthesis of AgNP-containing MIP membranes from the monomer mixtures containing both methacrylate monomers and oligomers as well as AgNOs solution in dimethylformamide enable the detection system. The AgNP were formed in the structure of the polymeric membranes during the pre-heating step followed by the UV-initiated polymerization. The highly selective sensor elements where MIP membranes are combined with the Ag nanostructures as signal amplifiers (LSPR-MIP nanochips) can be stored separately and used with a simple optical recognition system as selective elements of point-of-care sensor devices, even in in-field conditions. Composite plasmonic membranes provide both highly selective recognition of the target toxin and the generation of an enhanced optical signal, allowing aflatoxin B1 detection at ultralow concentrations.
A sensor chip comprising a MIP membrane or thin film having embedded silver nanoparticles (AgNP), wherein the MIP or thin film exhibits a binding affinity for a fluorophore of interest whereby the AgNP in the MIP enhances the fluorescence of the fluorophore upon application of UV irradiation.
A sensor chip comprising a MIP membrane or thin film having embedded nanoparticles selected from the group consisting of silver nanoparticles, gold nanoparticles, copper nanoparticles and aluminum nanoparticles, wherein the MIP or thin film exhibits a binding affinity for a fluorophore of interest whereby the nanoparticle in the MIP enhances the fluorescence of the fluorophore upon application of UV irradiation.
A method for the synthesis of AgNP-containing MIP membranes from monomer mixtures comprising the steps of providing a solution containing methacrylate monomers or oligomers and AgNO3 in dimethylformamide, forming the AgNP in the structure of the polymeric membranes during the pre-heating step and polymerzing the solution using UV-initiated polymerization.
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Dependencies are inferred from the claim text.