Capture compositions

A composition comprising:

• (a) a solid phase;
• (b) a molecular net capable of binding to at least one analyte and coupled to at least a portion of a surface of the solid phase, the molecular net comprising more than one layer, which more than one layers comprise at least a first layer and a second layer, and each of the layers comprises capture molecules capable of specifically binding to the analyte(s);
• and,
• (c) at least two different linker molecules,
• wherein:
• the capture molecules are coupled to each other within each layer by the at least two different linker molecules,
• the molecular net is an internally covalently-linked three dimensional matrix,
• the capture molecules within each layer can be of the same or different types,
• the capture molecules of one layer can be of the same or different types as the capture molecules of another layer, and
• the capture molecules comprise one or more antibodies directed to the analyte.

A method of measuring a quantity of an analyte in a sample, the method comprising:

• providing one or more devices each comprising a solid phase and a molecular net covering at least a portion of a surface of the solid phase, the molecular net including capture molecules of at least one type coupled to each other by linker molecules of a plurality of types to form a covalently-linked multi-layered three-dimensional matrix, the capture molecules configured to bind to the analyte;
• exposing the devices to the sample;
• and
• allowing at least a portion of the analyte to bind to the capture molecules of the molecular nets of the devices.