US 2025/0147010 A1 · DETECTION AGENT, DETECTION SYSTEM AND METHOD THEREOF

Abstract

A detection agent, a detection system, and a method thereof are provided. The detection agent includes magnetic barcode beads. Each of the magnetic barcode beads has different two-dimensional edge and is conjugated with a corresponding protein. The protein includes a receptor binding domain of a spike protein or a nucleocapsid protein from a virus or a variant of the virus. The detection system includes the detection agent and a barcode bead fluorescence reader to read a fluorescence signal generated by each of the magnetic barcode beads. The method includes the steps of adding a serum of the subject to the detection agent followed by adding a fluorescently labeled anti-human immunoglobulin antibody and reading a fluorescence signal generated by each of the magnetic barcode beads and discriminating each of the magnetic barcode beads by the barcode bead fluorescence reader to quantify the anti-human immunoglobulin antibody.

Claims18

Dependent claims

A detection agent for profiling a humoral response in a subject, comprising magnetic barcode beads, wherein each of the magnetic barcode beads has different two-dimensional edge and each of the magnetic barcode beads is conjugated with a corresponding protein;

wherein the protein comprises a receptor binding domain of a spike protein or a nucleocapsid protein from a virus or a variant of the virus;
wherein the receptor binding domain of the spike protein of the virus comprises an amino acid sequence of SEQ ID NO:
1;
the receptor binding domain of the spike protein of the variant of the virus comprises an amino acid sequence of SEQ ID NO:
2, SEQ ID NO:
3, SEQ ID NO:
4, SEQ ID NO:
5, SEQ ID NO:
6, SEQ ID NO:
7, SEQ ID NO:
8, or SEQ ID NO:
9.

9 more elements

Dependencies are inferred from the claim text.

Detection agent, detection system and method thereof

Life of this patent

Detection agent, detection system and method thereof

Life of this patent

Abstract

Claims18