Methods comprising therapeutic compounds and in vitro mammalian skin
AssigneeAmgen Inc.
InventorsMarisa K. JOUBERT, Nathan H. JOH, Joshua M. TOKUDA, John FERBAS, Jiansong XIE, Dong XIANG
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Abstract
Method for investigating subcutaneous administration of a therapeutic compound product comprising providing a live in vitro mammalian skin sample comprising a dermal layer and epidermal layer, administering a therapeutic compound product to the interface between the dermal layer and the epidermal layer and analysing the skin sample.
Claims41
A method of selecting a condition of administration for a therapeutic compound product for subcutaneous administration, the method comprising;
- providing a live in vitro mammalian skin comprising a dermal layer and epidermal layer that define an interface therebetween;
- administering a therapeutic compound product to the interface under a condition of administration;
- detecting a physiochemical and/or clinical characteristic of the therapeutic compound product in the interface;
- and
- selecting or rejecting the condition of administration for further development based on the detected physiochemical and/or clinical characteristic of the therapeutic compound.
A method of determining an impact of a molecular attribute of a therapeutic compound on a physiochemical and/or clinical characteristic of the therapeutic compound after subcutaneous administration, the method comprising;
- providing a live in vitro mammalian skin comprising a dermal layer and epidermal layer that define an interface therebetween;
- administering a therapeutic compound product comprising a molecular attribute to the interface;
- and
- detecting a physiochemical and/or clinical characteristic of the therapeutic compound in the interface.
A method of detecting a change in a molecular attribute of a therapeutic compound after subcutaneous administration, the method comprising;
- providing a live in vitro mammalian skin comprising a dermal layer and epidermal layer that define an interface therebetween;
- administering the therapeutic compound comprising the attribute to the interface, wherein the therapeutic compound comprises a first state of a molecular attribute;
- and
- detecting a second state of the molecular attribute of the therapeutic compound in the subcutaneous space.
Dependencies are inferred from the claim text.