Multi-depth spiral milli fluidic device for whole mount zebrafish antibody staining

A system for separating and retaining particles, the system comprising:

• (a) a fluidic chip with an arcuate channel with an outer wall and an inner wall, said arcuate channel forming a single loop spiral, said inner wall of said channel defining a central fluid collection reservoir, said arcuate channel open at one end to the central fluid collection reservoir, said inner wall of said arcuate channel having a plurality of particle traps;
• at least one fluid inlet fluidly coupled to said arcuate channel;
• and a fluid outlet fluidly coupled to said central fluid collection reservoir;
• (b) a pump;
• (c) a fluidic circuit between the pump and the fluid outlet of the central fluid collection reservoir of the fluidic chip;
• and
• (d) a controller configured to control actuation of the pump.

A method for particle separations in a fluid flow, the method comprising:

• (a) providing a fluidic chip with an arcuate annular channel defined by an outer wall and an inner wall, said channel open at one end to a central fluid collection reservoir, said inner wall of said channel having a plurality of particle traps, at least one fluid inlet fluidly coupled to said arcuate channel;
• and a fluid outlet inlet fluidly coupled to said central fluid collection reservoir;
• (b) flowing a fluid with particles for separation through said fluid inlet and arcuate channel;
• and
• (c) trapping individual particles within said particle traps from the fluid flow by controlling the flow of fluid into the channel and out of the collection reservoir.