Method for Calibrating an Analysis System for Lab-on-a-Chip Cartridges

Abstract

A method for calibrating measuring channels of an analysis system for lab-on-a-chip cartridges by way of a calibration cartridge is disclosed. The method includes filling a sample chamber of the calibration cartridge with a fluorophore-free solution, measuring the background fluorescence intensity for each of the measuring channels, pumping the solution within the microfluidic circuit and back into the sample chamber, measuring the background fluorescence intensity again for each measuring channel, averaging the measured background fluorescence intensities to give an averaged background fluorescence intensity, filling the sample chamber with a dye solution having a defined concentration of at least one fluorophore, measuring the fluorescence intensity of the dye solution for each of the measuring channels, pumping the solution within the microfluidic circuit and back into the sample chamber, measuring the fluorescence intensity of the dye solution again for each of the measuring channels, averaging the measured fluorescence intensities of the dye solution to give averaged fluorescence intensities for each of the measuring channels, and calculating the calibration matrix from the averaged fluorescence intensity of the dye solution and, preferably while taking into account of a preferably averaged background fluorescence intensity for each of the measuring channels.

Dependencies are inferred from the claim text.