Aberrant viral rna detection using cas13
AssigneeThe Trustees of Princeton University
InventorsCaitlin Lamb, Arend Jan Wouter te Velthuis, Cameron Myhrvold
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Abstract
Disclosed is a method for quantitative detection of a small, aberrant viral nucleic acid. The method includes combining a detection mixture with a sample, and allowing a reaction with the test mixture to begin. The sample includes an RNA sample. The detection mixture includes a Cas13 enzyme, a reporter, a sequence-specific CRISPR RNA (crRNA), and a buffer. The method includes determining a quantitative value representative of the reporter in the test mixture, where the quantitative value is a measure of an amount of target RNA present in the RNA sample.
Claims19
A composition of matter, comprising:
- a Cas13 enzyme;
- a reporter;
- a sequence-specific CRISPR RNA (crRNA);
- and
- a buffer.
A method for quantitative detection of a small, aberrant viral nucleic acid, comprising:
- forming a test mixture by combining a detection mixture with a sample, the sample comprising an RNA sample, the detection mixture comprising:
- a Cas13 enzyme;
- a reporter;
- a sequence-specific CRISPR RNA (crRNA);
- and
- a buffer;
- allowing a reaction within the test mixture to begin;
- and
- determining a quantitative value representative of the reporter in the test mixture, where the quantitative value is a measure of an amount of target RNA present in the RNA sample.
Dependencies are inferred from the claim text.