Universal early cancer diagnostics
AssigneesPresident and Fellows of Harvard College, Dana-Farber Cancer Institute, Inc.
InventorsAlexander Meissner, Zachary D. Smith, Franziska Michor, Jiantao Shi
Life of this patent
Estimating the term…
Abstract
Methods for quantifying DNA methylation that may be utilized for screening for diseases (e.g., cancer), diagnosing diseases (e.g., cancer type), monitoring progression of a disease, and monitoring response to a therapeutic treatment.
Claims17
A method of preparing a dataset of sequencing reads for at least one CpG Island (CGI) for use in detecting circulating tumor DNA (ctDNA) from a patient sample, comprising:
- a. treating cell free DNA (cfDNA) obtained from the patient sample with bisulfite;
- b. amplifying the at least one CGI from the bisulfite-treated cfDNA, wherein the at least one CGI is a CGI hypermethylated in human placenta compared to human embryonic stem cells, or is orthologous to a CGI hypermethylated in mouse extraembryonic ectoderm (ExE) compared to epiblast;
- c. sequencing the amplified DNA using next-generation sequencing (NGS) to generate a dataset comprising a plurality of sequencing reads for the at least one CGI.
A method of disrupting methylation of CpG islands comprising reducing expression of Eed, Dnmt1, Dnmt3l, Dnmt3b, or PRC2.
Dependencies are inferred from the claim text.