Method for transposase-mediated spatial tagging and analyzing genomic dna in a biological sample

A method for determining the spatial location of a nucleic acid in a tissue sample, the method comprising:

• (a) contacting the tissue sample with an array comprising a plurality of capture probes affixed thereon, wherein a capture probe of the plurality of capture probes comprises (i) a spatial barcode and (ii) a capture domain;
• (b) permeabilizing the tissue sample;
• (c) contacting a transposome with the permeabilized tissue sample to insert transposon end sequences into the nucleic acid, thereby generating a fragmented nucleic acid sequence, and adding to an end of the fragmented nucleic acid sequence a first adapter comprising a sequence substantially complementary to a sequence of the capture domain;
• (d) hybridizing the first adapter of the fragmented nucleic acid sequence to the capture domain of the capture probe;
• and
• (e) determining the spatial barcode sequence or a complement thereof, and the fragmented nucleic acid sequence adjacent to the first adapter and the transposon end sequence, or a complement thereof, thereby determining the spatial location of the nucleic acid in the tissue sample.