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US 2025/0146066 A1Published application
Nucleotides with a 3' aom blocking group
AssigneeIllumina Cambridge Limited
InventorsAntoine FRANCAIS, Elena CRESSINA, Adam CULLEY, Angelica MARIANI, Xiaolin WU, Xiaohai LIU
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Abstract
Embodiments of the present disclosure relate to nucleotide molecules with a 3′ AOM blocking group. Also provided herein are methods to prepare such nucleotide molecules, and the uses of fully functionalized nucleotides containing the 3′-OH blocking group for sequencing applications.
Claims20
A kit for sequencing, comprising:
- a first type of nucleotide comprising a 2′ deoxyribose having a removable 3′-OH blocking group forming a structure
- covalently attached to the 3′-carbon atom of the 2′ deoxyribose, wherein:
- each of R1a, R1b, R2a and R2b is independently H, C1-C6 alkyl, C1-C6 haloalkyl, cyano, or halogen;
- and
- R3 is C2-C6 alkenyl or C2-C6 alkynyl, each optionally substituted with one or more substituents independently selected from the group consisting of halogen, C1-C6 alkyl, C1-C6 haloalkyl and combinations thereof.
2 more elements
Dependencies are inferred from the claim text.