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US 2025/0146062 A1Published application
Multiplex Amplification Detection Assay II
OwnerHatim T. Allawi, William G. Weisburg +4inventors
InventorsHatim T. Allawi, William G. Weisburg, Graham P. Lidgard, Michael W. Kaiser, Abram M. Vaccaro, Gracie Shea
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Abstract
Provided herein is technology relating to the amplification-based detection of bisulfite-treated DNAs and particularly, but not exclusively, to methods and compositions for multiplex amplification of low-level sample DNA prior to further characterization of the sample DNA. The technology further provides methods for isolating DNA from blood or blood product samples, e.g., plasma samples.
Claims18
-20. (canceled)
A method of analyzing a sample comprising bisulfite-treated DNA for multiple target nucleic acids, comprising:
- (a) partitioning the sample into a plurality of different detection assay reaction mixtures, each mixture of the plurality of different detection assay reaction mixtures comprising flap oligonucleotides and a primer pair that binds to a target nucleic acid;
- and
- (b) conducting a plurality of detection assays on the detection assay reaction mixtures, wherein the detection assays are PCR-flap assays,
- wherein one or more of the flap oligonucleotides comprise a target-specific region of at least 13 bases in length.
1 more element
Dependencies are inferred from the claim text.