System for nucleic acid analysis

A system (1) for PCR analysis, the system (1) comprising:

• a test card receiver (4) configured to receive a test card (6), wherein the test card (6) comprises a sample portion (8) having a first side (10) and a second side (12) opposite the first side (10), wherein the sample portion (8) of the test card (6) is configured to hold a sample for PCR,
• a temperature adjuster (14) configured to adjust a temperature of the sample portion (8) of the test card (6) over a plurality of temperature cycles, wherein each temperature cycle is between a first temperature and a second temperature different than the first temperature, wherein the temperature adjuster (14) comprises a surface (16) configured to be in contact with the first side (10) of the sample portion (8) of the test card (6),
• wherein the test card receiver (4) comprises a spring member (18) configured to push the sample portion (8) of the test card (6) towards the surface (16) of the temperature adjuster (14), and
• a monitoring arrangement (20) configured to monitor the sample portion (8) of the test card (6) over the plurality of temperature cycles, the monitoring arrangement (20) comprising:
• at least one light sensor (22),
• an illumination system (24) configured to illuminate the second side (12) of the sample portion (8) with light having wavelengths within at least three excitation wavelengths ranges,
• an emission filter (26) configured to transmit light having wavelengths within at least three emission wavelength ranges, and
• collection optics (28) configured to collect light emanating from the second side (12) of the sample portion (8) of the test card (6) and transmitted through the emission filter (26), and wherein the collection optics (28) is further configured to direct the collected light towards the light sensor (22) such that at least a portion of the collected light impinges on the light sensor (22),
• wherein the light sensor (22) is configured to generate at least one signal(S), wherein each signal(S) pertains to light impinging on the light sensor (22) after a unique number of temperature cycles of the plurality of temperature cycles.