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US 2025/0146049 A1Published application
Methods for preparing a sample for nucleic acid amplification
AssigneeIllumina Cambridge Limited
InventorsLouise Fraser, Paula Kokko-Gonzales, Andrew Slatter
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Abstract
Presented are methods and compositions for preparing samples for amplification and sequencing. Particular embodiments relate to methods of preparing nucleic acid-containing cellular samples for library amplification, wherein the methods include lysing cells of the sample to form a lysate, amplifying the nucleic acids from the lysed samples, exposing the amplified nucleic acids to a solid surface, and clonallyr amplifying the amplified nucleic acids to generate clusters.
Claims19
A flow cell comprising a solid surface comprising:
- immobilized amplification primers;
- proteinase K;
- paraffin;
- and
- one or more of formalin, cellular components, protein, extracellular matrix components, collagen, or tissue debris.
2 more elements
Dependencies are inferred from the claim text.