Method of preparing ibuprofen by enzymatic conversion and a modified polypeptide thereof
OwnerNaveen K. Kulkarniinventor
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Abstract
The present invention relates to an enzymatic conversion of aldehyde to carboxylic acid for the preparation of ibuprofen. In particular, the present disclosure provides a method of preparation of 2-(4-isobutylphenyl) propanoic acid that is ibuprofen by enzymatic conversion of 2-(4-isobutylphenyl) propanal that is ibuprofen aldehyde to 2-(4-Isobutylphenyl) propanoic acid that is ibuprofen in presence of an oxidoreductase enzyme with high conversion efficiency.
Claims21
A method of preparation of 2-(4-isobutylphenyl) propanoic acid by enzymatic conversion of 2-(4-isobutylphenyl) propanal to 2-(4-isobutylphenyl) propanoic acid by an oxidoreductase enzyme in the presence of a buffer.
A method of preparation of alkyl substituted phenyl carboxylic acids of the formula I using the alkyl substituted phenyl aldehydes of the formula II as substrate using an oxidoreductase enzyme, a cofactor and a buffer.
- wherein R is an alkyl group of the formula CnH2n+1 with n=0-4 and R-group substitutions can be hydrogen, methyl, ethyl, propyl, isopropyl, butyl, tert-butyl, isobutyl.
A modified oxidoreductase polypeptide comprising the SEQ ID NO:
- 11, SEQ ID NO:
- 12, SEQ ID NO:
- 13, SEQ ID NO:
- 14, and/or SEQ ID NO:
- 15, wherein the recombinant polypeptides comprise amino acid substitutions from the group consisting of:
- a. Asp85 substituted with Arg or any polar, aliphatic, basic amino acid.
- b. Glu483 substituted with Arg or any polar, aliphatic, basic amino acid.
- c. Asp490 substituted with Arg or any polar, aliphatic, basic amino acid.
- d. Glu494 substituted with Arg or any polar, aliphatic, basic amino acid.
Dependencies are inferred from the claim text.