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AssigneeMax-Planck-Gesellschaft zur Foerderung der Wissenschaften e.V.
InventorsMelina SCHUH, Chun SO
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It has been surprisingly found that human oocytes lack the important spindle-associated protein KIFC1/HSET. The application describes a method stabilising the human spindle with KIFC1/HSET. Specifically, the method relates to introducing (i) KIFC1/HSET protein or (ii) mRNA encoding KIFC1/HSET into a human oocyte. Furthermore, the application relates to a non-naturally occurring human oocyte, wherein a (i) KIFC1/HSET protein or (ii) KIFC1/HSET mRNA has been introduced into a naturally occurring human oocyte thereby obtaining the non-naturally occurring oocyte. Additionally, the application relates to a (i) KIFC1/HSET protein or (ii) mRNA encoding the KIFC1/HSET for use in a method of lowering the probability of having a disorganized and/or a multipolar spindle during mitosis in a human zygote by introducing the (i) KIFC1/HSET protein or (ii) mRNA encoding the KIFC1/HSET into the human zygote. Moreover, the application discloses a complex comprising (i) a KIFC1/HSET protein and (ii) a human meiotic spindle or a human mitotic spindle, wherein the KIFC1/HSET protein has been introduced into a human oocyte or zygote.
1-21. (canceled)
An in vitro method for use in assisted reproductive technology of introducing (i) KIFC1/HSET protein or (ii) mRNA encoding KIFC1/HSET into a human oocyte.
A non-naturally occurring human oocyte, wherein the non-naturally occurring human oocyte is obtained by introduction of a suitable amount of (i) KIFC1/HSET protein or (ii) KIFC1/HSET mRNA into a naturally occurring human oocyte, wherein (i) the KIFC1/HSET protein or (ii) KIFC1/HSET protein expressed from the mRNA encoding the KIFC1/HSET stabilizes the meiotic spindle of the oocyte.
A method of lowering the probability of aneuploidy by introducing (i) KIFC1/HSET protein or (ii) mRNA encoding the KIFC1/HSET into a human zygote.
A complex in assisted reproductive technology comprising (i) a KIFC1/HSET protein and (ii) a human meiotic spindle or a human mitotic spindle, obtained by introduction of a suitable amount of the KIFC1/HSET protein into a human oocyte or zygote by an in vitro method, wherein the KIFC1/HSET protein has been introduced by microinjection and/or electroporation, wherein the complex is detectable by fluorescence microscopy, wherein the KIFC1/HSET protein stabilizes the spindle, and wherein the stabilized spindle is a bipolar spindle.
Dependencies are inferred from the claim text.