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US 2025/0146010 A1Published application
Compositions and methods for soybean plant transformation
AssigneeInari Agriculture Technology, Inc.
InventorsTEJINDER KUMAR MALL, CHRISTOPHER ALEXANDER BAGLEY, RHODA ARABA TAWIAH BREW-APPIAH, LARISSA ARRAIS GUIMARAES
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Abstract
Chimeric GRF-GIF polypeptides that can be used to improve the capacity of plant cells to regenerate embryogenic plant tissues, plant organs, and whole plants are disclosed. Also disclosed are plant cells comprising the chimeric polypeptides and related methods for improving the capacity of the plant cells to regenerate embryogenic plant tissues, plant organs, and whole plants.
Claims39
A composition comprising:
- (i) a first recombinant polynucleotide comprising:
- (a) polynucleotide encoding a chimeric GRF4-GIF1 (cGRF4-GIF1) polypeptide, optionally wherein the GRF4-GIF1 polypeptide comprises a chimeric dicot plant GRF4-GIF1 (cdpGRF4-dpGIF1) polypeptide or a chimeric GmGRF4-GmGIF1 (cGmGRF4-GmGIF1) polypeptide, said cGmGRF4-GmGIF1 polypeptide comprising from its amino terminus (N-terminus) to its carboxy terminus (C-terminus) a GmGF4 polypeptide comprising an amino acid sequence having at least 92%, 95%, 98%, or 99% sequence identity across the entire length of SEQ ID NO:
- 1 and a GmGIF1 polypeptide comprising an amino acid sequence having at least 85%, 90%, 95%, 98%, or 99% sequence identity across the entire length of SEQ ID NO:
- 2;
- and (b) a terminator which is operably linked to said polynucleotide;
- and
- (ii) a second recombinant polynucleotide comprising a promoter which is operably linked to a polynucleotide encoding an acetohydroxyacid synthase (AHAS) which confers resistance to an AHAS inhibitor and a terminator which is operably linked to said polynucleotide.
4 more elements
Dependencies are inferred from the claim text.