US 2025/0146009 A1Published application

Plant grain size regulation gene and use thereof

AssigneeCapital Normal University

InventorsLigeng MA, Ying CAO, Jing WU

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Abstract

Disclosed are a plant grain size regulation gene and use thereof, which belong to the technical field of biology. PCFS gene mutants in different crops are obtained by gene editing, and by means of experiments and statistical analysis, it is found that the mutants have a phenotype of enlarged plant grain and part of the mutants have a phenotype of improved stress resistance. The provided gene, mutants, and method for use same are beneficial to increase the crop yield and improve the quality and enhance the resistance of plants to an adverse environment, provide gene resources and technical support for cultivating new plant varieties with large grain weight and high stress resistance, and thereby have important significance and application value for improvement of agronomic traits of crops and breeding of high-yield and high stress-resistant molecules.

Claims16

Dependent claims

An isolated polynucleotide of a gene mutant, wherein a plant containing the gene mutation has bigger grains, and wherein said gene has an isolated polynucleotide selected from one of the sequences in the following group:

(a) a nucleotide sequence with a gene ID of BnaA04G0223600WE, BnaA05G0075100WE, BnaA09G0222400WE, BnaC04G0102200WE, BnaC04G0535800WE or BnaC09G0254600WE in Brassica napus;
(b) a nucleotide sequence with a gene ID of Glyma.03G191200, Glyma.10G066300, Glyma.10G251100, Glyma.19G191800, Glyma.19G191900 or Glyma.20G142500 in soybean;
(c) a nucleotide sequence with a gene ID of LOC_Os08g08830 or LOC_Os09g39270 in rice;
or
(d) a nucleotide sequence with a gene ID of Zm00001d000023, Zm00001d005350, Zm00001d019856 or Zm00001d049442 in maize;
or
(e) an isolated polynucleotide capable of hybridizing with any one of the isolated polynucleotide of (a) to (d) under a stringent condition;
(f) a nucleotide sequence having at least 85%, 90%, 95% or more sequence similarity with a full length of any one of the isolated polynucleotide of (a) to (e), and having a function of making a plant have bigger grains after mutation in the plant;
or
(g) a nucleotide sequence complementary to any one of the nucleotide sequences of (a) to (f).

7 more elements

A method for regulating agronomic traits of a plant, comprising mutating a PCFS family gene of the plant to make the plant have bigger grains, wherein said gene has a polynucleotide selected from one of the sequences in the following group:

(a) a nucleotide sequence with a gene ID of BnaA04G0223600WE, BnaA05G0075100WE, BnaA09G0222400WE, BnaC04G0102200WE, BnaC04G0535800WE or BnaC09G0254600WE in Brassica napus;
(b) a nucleotide sequence with a gene ID of Glyma.03G191200, Glyma.10G066300, Glyma.10G251100, Glyma.19G191800, Glyma.19G191900 or Glyma.20G142500 in soybean;
(c) a nucleotide sequence with a gene ID of LOC_Os08g08830 or LOC_Os09g39270 in rice;
or
(d) a nucleotide sequence with a gene position ID of Zm00001d000023, Zm00001d005350, Zm00001d019856 or Zm00001d049442 in maize;
(e) a nucleotide sequence capable of hybridizing with any one of the nucleotide sequences of (a) to (d) under a stringent condition;
(f) a nucleotide sequence having at least 85%, 90%, 95% or more sequence similarity with a full length of any one of the nucleotide sequences of (a) to (e), and having a function of making a plant have bigger grains after mutation in the plant;
or
(g) a DNA sequence complementary to any one of the nucleotide sequences of (a) to (f).

6 more elements

Dependencies are inferred from the claim text.

Facts

Application: 19/011,969Filed 7 Jan 2025
Priority: CN 202210999291.419 Aug 2022
Published: 8 May 2025
Term ends: Not estimatedThe source records don’t support an estimate.

Classification21 codes

C12N 15/8261
Phenotypically and genetically modified plants via recombinant DNA technology: with agronomic traits, e.g. crop yield
A01H 5/00
Angiosperms, i.e. flowering plants, characterised by their plant parts
A01H 5/10
Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy: seeds
A01H 6/20
Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy: brassicaceae, e.g. canola, broccoli or rucola

Family

Application19/011,969Continuation
US 2025/0146009 A1This patent

From the US filing history. Foreign family members aren’t listed.

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