Methods and compositions for trichome removal
AssigneePairwise Plants Services, Inc.
InventorsBrian Charles Wilding Crawford, Julius Mojica, Thomas J. Poorten
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Abstract
This invention relates to compositions and methods for modifying GLABRA2 (GL2) genes in Brassica plants. The invention further relates to Brassica plants and parts thereof having a phenotype of modified trichomes, a reduced number of trichomes, or no trichomes using the methods and compositions of the invention.
Claims29
A Brassica plant or part thereof comprising at least one mutation in an endogenous GLABRA2 (GL2) gene encoding a GL2 polypeptide, wherein the endogenous GL2 gene comprises (a) a nucleotide sequence having at least 80% sequence identity to any one of SEQ ID NOs:69, 70, 72, 73, 106, 107, 109, 110, 112, 113, 115, 116, 118, 119, 121, 122, 124, 125, 127, 128, 130, 131, 133 and/or 134, or comprises a region having at least 80% sequence identity to a nucleotide sequence of any one of SEQ ID NOs:75-102, or (b) encodes a polypeptide having at least 80% sequence identity to any one of SEQ ID NOs:71, 74, 108, 111, 114, 117, 120, 123, 126, 129, 132, and/or 135.
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A method for producing a Brassica plant or part thereof comprising at least one cell having a mutated endogenous GLABRA2 (GL2) gene, the method comprising contacting a target site in an endogenous GL2 gene in the Brassica plant or plant part with a nuclease comprising a cleavage domain and a nucleic acid binding domain, wherein the nucleic acid binding domain binds to a target site in the endogenous GL2 gene, wherein the endogenous GL2 gene
- (a) comprises a sequence having at least 80% sequence identity to any one of the nucleotide sequences of SEQ ID NOs:69, 70, 72, 73, 106, 107, 109, 110, 112, 113, 115, 116, 118, 119, 121, 122, 124, 125, 127, 128, 130, 131, 133 and/or 134;
- (b) comprises a region having at least 80% identity to any one of SEQ ID NOs:75-102;
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- (c) encodes an amino acid sequence having at least 80% sequence identity to any one of SEQ ID NOs:71, 74, 108, 111, 114, 117, 120, 123, 126, 129, 132, and/or 135, thereby producing the Brassica plant or part thereof comprising at least one cell having a mutation in the endogenous GL2 gene.
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A guide nucleic acid that binds to a target site in a GLABRA2 (GL2) gene, wherein the target site is in a region of the GL2 gene having at least 80% sequence identity to any one of SEQ ID NOs:75-102.
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Dependencies are inferred from the claim text.