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AssigneeThe U.S.A., as represented by the Secretary, Department of Health and Human Services
InventorsRobert D. Daniels, Jan-Willem de Gier, Tahir H. Malik
Estimating the term…
Recombinant nucleic acid molecules engineered for efficient propagation of a heterologous DNA sequence (such as a heterologous viral gene) that is toxic in E. coli are described. Influenza virus hemagglutinin (HA) and neuraminidase (NA) genes were used as exemplary toxic heterologous DNA sequences. Plasmids that included a heterologous DNA positioned between bacterial regulatory elements (such as lac operator sequences and/or terminator sequences) exhibited decreased gene transcription in E. coli and increased stability of the heterologous DNA while also retaining the property of inducible expression in E. coli.
A recombinant nucleic acid molecule, comprising in the 5′ to 3′ direction:
(canceled)
A plasmid, comprising in the 5′ to 3′ direction:
A plasmid, comprising in the 5′ to 3′ direction:
Dependencies are inferred from the claim text.