Oligonucleotides with ethylene glycol modification
AssigneeUniversity of Massachusetts
InventorsAnastasia Khvorova, Daniel O'Reilly, Vignesh Narayan Hariharan, Raymond Furgal, Katherine Gross
Life of this patent
Estimating the term…
Abstract
The disclosure relates to compositions, systems, and methods comprising ethylene glycol chemical modifications on oligonucleotides to modulate silencing activity and improve stability.
Claims39
An RNA molecule comprising a 5′ end and a 3′ end, wherein the RNA molecule comprises at least one ethylene glycol modification to one or both of the 5′ end and the 3′ end, wherein the at least one ethylene glycol modification comprises 1-20 ethylene glycol units.
4-7. (canceled)
(canceled)
(canceled)
15-18. (canceled)
(canceled)
23-44. (canceled)
(canceled)
48-50. (canceled)
55-56. (canceled)
58-64. (canceled)
(canceled)
A double stranded RNA (dsRNA), comprising an antisense strand and a sense strand, each strand with a 5′ end and a 3′ end, wherein the antisense strand comprises at least one ethylene glycol modification to one or both of the 5′ end and the 3′ end, wherein the at least one ethylene glycol modification comprises 1-20 ethylene glycol units.
69-92. (canceled)
A double stranded RNA (dsRNA), comprising an antisense strand and a sense strand, each strand with a 5′ end and a 3′ end, and at least one single stranded nucleotide overhang of 2-5 nucleotides, wherein the at least one single stranded nucleotide overhang comprises at least one ethylene glycol modification.
94-96. (canceled)
A double stranded RNA (dsRNA), comprising an antisense strand and a sense strand, each strand with a 5′ end and a 3′ end, wherein;
- the antisense strand comprises a chemical modification pattern of any one of the chemical modification patterns provided in Tables 2-6;
- or
- the sense strand comprises a chemical modification pattern of any one of the chemical modification patterns provided in Tables 2-3.
(canceled)
100-101. (canceled)
Dependencies are inferred from the claim text.