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US 2025/0145989 A1Published application
Method for detecting target nucleic acids in sample
AssigneeSeegene, Inc.
InventorsSuk-Tae KWON, Sung Suk CHO, Sunghui KIM, Jeongeun HAN
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Abstract
A method for detecting target nucleic acids in a sample is disclosed. The method can rapidly detect target nucleic acids and has simplified protocols. Therefore, the method can be effectively used by large hospitals, entrusted testing institutions, research laboratories, and the like at which rapid molecular diagnosis for a large number of samples is required.
Claims24
A method for detecting a target nucleic acid in a sample, comprising:
- (a) preparing a reaction mixture by mixing the sample with a composition for detecting the target nucleic acid;
- wherein the composition comprises:
- (i) an oligonucleotide capable of hybridizing with the target nucleic acid, (ii) a thermostable Sso7d fusion DNA polymerase and (iii) an antibody specifically bound to the thermostable Sso7d fusion DNA polymerase for inhibiting its activity,
- (b) incubating the reaction mixture at 65° C. or higher to induce denaturation of the antibody;
- wherein the denaturation of the antibody restores the activity of the thermostable Sso7d fusion DNA polymerase;
- and
- (c) amplifying the target nucleic acid in the reaction mixture.
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Dependencies are inferred from the claim text.