Methods for in vivo delivery and detection of clustered regularly interspaced short palindromic repeat systems utilizing baculovirus vector-magnetic nanoparticle complexes

A method for promoting an immune response in a subject, comprising:

• administering a baculovirus vector (BV)-magnetic nanoparticle (MNP) (BV-MNP) complex carrying a clustered regularly interspaced palindromic repeat (CRISPR) associated protein 9 (Cas9) and a guide RNA to the subject, the guide RNA having homology to an immune checkpoint gene impeding the immune response in the subject;
• wherein administration of the BV-MNP complex carrying the Cas9 and the guide RNA inhibits the immune checkpoint gene.

A method for inhibiting tumor growth in a subject, comprising:

• administering a baculovirus vector (BV)-magnetic nanoparticle (MNP) (BV-MNP) complex carrying a clustered regularly interspaced palindromic repeat (CRISPR) associated protein 9 (Cas9) and a guide RNA to tumor tissue in the subject, the guide RNA having homology to an immune checkpoint gene impeding infiltration of at least one of lymphocytes and dendritic cells into the tumor tissue;
• wherein administration of the BV-MNP complex carrying the Cas9 and the guide RNA inhibits the immune checkpoint gene.