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US 2025/0145960 A1Published application
A method of derivation of mesenchymal stem cells from mammalian pluripotent stem cells
AssigneeThe University of Hong Kong
InventorsZhongjun Zhou, Wei Jin
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Abstract
The subject invention pertains to methods of derivation of MSCs from both human or other mammal iPSCs and embryonic stem cells (ESCs) via a temporal induction of neural ectoderm in chemically defined media. In certain embodiments, the methods use a two-stage culture.
Claims19
A method of producing Mesenchymal stem cells (MSCs) from mammalian pluripotent stem cells, the method comprising:
- culturing the mammalian pluripotent stem cells for a first time period in a first medium comprising a cell basal medium, a cell culture supplement, a non-essential amino acids solution, sodium pyruvate, insulin, transferrin, selenium, L-ascorbic acid, an antibiotic solution, a GSK-3 inhibitor, and an inhibitor of activin receptor-like kinase receptors.
Dependencies are inferred from the claim text.