Generating Vasculogenic Cell Populations
AssigneeWisconsin Alumni Research Foundation
InventorsIgor Slukvin, Akhilesh Kumar
Life of this patent
Estimating the term…
Abstract
The present invention relates generally to methods and compositions useful for therapeutic vascular tissue engineering. In particular, the present invention provides methods for generating substantially pure populations of vasculogenic cells from human mesenchymal progenitor, and methods and compositions for clinical applications in the field of regenerative medicine.
Claims9
1-22. (canceled)
A method for obtaining an expanded isolated population of primate vasculogenic cells for vascular tissue engineering and for pharmaceutical use, the method comprising:
- obtaining mesenchymal stem cells from a mesenchymoangioblasts (MAB)-derived colony of mesenchymal progenitors,
- expanding the population of mesenchymal stem cells in vitro, and
- contacting the expanded MSC population to a culture medium comprising an effective amount of sphingosylphosphorylcholine (SPC) and an effective amount of Transforming Growth Factor beta (TGFβ) to promote differentiation of the contacts MSC population into vasculogenic cells, wherein the vasculogenic cells are smooth muscle cells.
A method for obtaining an expanded isolated population of primate vasculogenic cells for vascular tissue engineering and for pharmaceutical use, the method comprising:
- obtaining mesenchymal stem cells from a mesenchymoangioblasts (MAB)-derived colony of mesenchymal progenitors,
- expanding the population of mesenchymal stem cells in vitro,
- culturing a mesenchymal stem cell colony in a culture medium with FVF2 to obtain mesenchymal stem cell lines, and
- contacting the expanded MSC population to a culture medium comprising an effective amount of sphingosylphosphorylcholine (SPC) and an effective amount of Transforming Growth Factor beta (TGFβ) to promote differentiation of the contacts MSC population into vasculogenic cells, wherein the vasculogenic cells are smooth muscle cells.
Dependencies are inferred from the claim text.