Kit and method for extracting cell mitochondria in vitro
AssigneeShanghai Sixth People's Hospital
InventorsJunjie GAO, Changqing ZHANG, Yiyang MA
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AssigneeShanghai Sixth People's Hospital
InventorsJunjie GAO, Changqing ZHANG, Yiyang MA
Estimating the term…
The invention provides a kit and a method for extracting cell mitochondria in vitro. The kit comprises an extracting solution A, an extracting solution B, an extracting solution C and a storage solution, wherein the extracting solution A comprises EDTA (Ethylene Diamine Tetraacetic Acid), sodium lactate and sodium chloride, the extracting solution B is a heparin solution, the extracting solution C comprises mannitol, EDTA, sodium lactate and sodium chloride, and the storage solution comprises mannitol, EDTA, sodium lactate, succinic acid, sodium pyruvate and sodium chloride. The functions of mitochondria can be better maintained in vitro by utilizing the extracting solution and the storage solution, the interval time from mitochondria extraction to mitochondria application to the body of a patient is prolonged, and beneficial help is provided for clinical application of mitochondria transplantation.
A kit for extracting cell mitochondria in vitro, comprising:
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