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US 2025/0145954 A1Published application
Expansion of human hematopoietic stem cells from human yellow bone marrow
AssigneeUniversity of Massachusetts
InventorsTammy NGUYEN, Silvia CORVERA, Tiffany DESOUZA, Anand DESAI
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Abstract
Hematopoietic stem cells (HSC) are essential to developing a functional immune system, including monocyte-macrophage cells required for wound healing. Described herein are methods of using yellow bone marrow as a source of cells to generate hematopoietic stem cells. The methods include expansion of human hematopoietic stem cells by culture of human yellow bone marrow in 3-dimensional hydrogel and pro-angiogenic, stem cell-promoting medium.
Claims18
A method of obtaining a population of hematopoietic stem cells (HSCs), the method comprising:
- providing a sample comprising yellow bone marrow from a subject;
- dividing the sample into pieces each about 0.5-2 mm3, preferably about 1 mm3;
- embedding the pieces in a hydrogel with media for expansion of HSCs;
- maintaining the pieces in culture for a time sufficient for expansion of the HSCs, and isolating the HSCs from the hydrogel,
- thereby obtaining a population of HSCs.
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Dependencies are inferred from the claim text.