Compositions and methods for expanding lymphocytes
AssigneeStemcell Technologies Canada Inc.
InventorsHitesh ARORA, Mark BROWN
Life of this patent
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Abstract
Disclosed are media, supplements and methods for expanding mammalian lymphocytes or progenitors thereof, such as B cells, T cells, or NK cells. The disclosed media, supplements and methods may be used to expand mammalian lymphocytes or progenitors thereof plated as single cells, or at a clonal cell density. In some embodiments, the disclosed media, supplements and methods may also differentiate/activate the lymphocytes during expansion. The media, supplements and methods of this disclosure may be used in serum-free and feeder-free culture workflows.
Claims35
A cell culture media supplement for expanding mammalian lymphocytes or progenitors thereof, the supplement comprising:
- one or more of or two or more of:
- a ligand of CD40, a mixture of lipids, and one or more cytokines;
- and
- a diluent.
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A culture medium for expanding mammalian lymphocytes or progenitors thereof, the medium comprising:
- a basal medium;
- and
- one or more of or two or more of:
- a ligand of CD40, a mixture of lipids, and one or more cytokines.
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A method of expanding mammalian lymphocytes or progenitors thereof, the method comprising:
- seeding the lymphocytes or progenitors thereof into a culture environment comprising one or more of a ligand of CD40, a mixture of lipids, and one or more cytokines;
- and
- incubating the seeded lymphocytes or progenitors thereof in the culture environment for more than one day.
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Dependencies are inferred from the claim text.