Methods for Activation and Expansion of T Cells
AssigneeAlaunos Therapeutics, Inc.
InventorsDrew Caldwell DENIGER, Lenka Victoria HURTON, Donghyun JOO, Yaoyao SHI, An LU, Victor CARPIO, Matthew COLLINSON-PAUTZ
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Abstract
Methods for culturing and expanding lymphocytes using unconventional cytokines are provided. These methods include techniques for culturing and expanding lymphocytes using streamlined approaches, including approaches using agonists for stimulation, and approaches more suitable for clinical manufacturing. Compositions of expanded populations of lymphocytes are also provided.
Claims49
A method of expanding a population of electroporated T cells comprising culturing the electroporated T cells with a first culture medium comprising one or more cytokines.
A population of engineered T cells, wherein more than 10% of the engineered T cells in the population comprise an exogenous TCR or functional fragment thereof, and wherein more than 20% of the population of engineered T cells are CCR7+/CD45RO+.
A population of engineered T cells, wherein more than 10% of the engineered T cells in the population comprise an exogenous TCR or functional fragment thereof, and wherein more than 40% of the population of engineered T cells are CD95+/CD62L+.
A population of cells comprising a polycistronic expression cassette comprising:
- a. a first cistron comprising a polynucleotide sequence that encodes a fusion protein that comprises IL-15, or a functional fragment or functional variant thereof, and IL-15Rα, or a functional fragment or functional variant thereof;
- b. a second cistron comprising a polynucleotide sequence that encodes a TCR beta chain comprising a Vβ region and a Cβ region;
- and
- c. a third cistron comprising a polynucleotide sequence that encodes a TCR alpha chain comprising a Vα region and a Cα region.
Dependencies are inferred from the claim text.