Generation of pure retinal cells from human pluripotent stem cells
OwnerSu-Chun Zhang, Yunlong Taoinventors
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Abstract
Provided herein are methods for producing neuroectoderm and retinal progenitor cells from pluripotent stem cells, and compositions of retinal progenitor cells produced thereby.
Claims22
A method for producing neuroectoderm cells from human pluripotent stem cells (hPSCs) comprising the steps of treating hPSCs in cell culture in vitro with inhibitors of BMP or TGF-beta, alone or in combinations thereof, for no more than 6 days in chemically defined media.
A method for producing retinal progenitor cells from primitive neuroectoderm cells (PNCs) comprising the step of treating the PNCs in cell culture in vitro with BMP2, BMP4, or BMP7 for about 20 days.
A method for producing retinal progenitor cells from human pluripotent stem cells (hPSCs) comprising the steps of treating hPSCs in cell culture with a TGF-beta inhibitor for five days followed by treatment with BMP activator from day 6 to day 20.
Dependencies are inferred from the claim text.