Culture media for culturing pluripotent stem cells in suspension
AssigneeAccellta Ltd.
InventorsMichal AMIT, Itzchak ANGEL
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Abstract
Provided are defined culture media for culturing pluripotent stem cells in a suspension culture devoid of substrate adherence, the defined culture media comprising an effective amount of a protease inhibitor; a GSK3β inhibitor and at least one agent selected from the group consisting of a protease inhibitor and a WNT3A polypeptide; a WNT3A polypeptide and a stabilizing agent thereof with the proviso that said stabilizing agent is not a lipid vesicle; and/or a GSK3β inhibitor, with the proviso that the medium is devoid of an ERK1/2 inhibitor. Also provided are cell cultures and methods of suing same.
Claims20
A defined culture medium comprising an effective amount of a protease inhibitor and at least one of a defined lipid mixture or a serum replacement, wherein said protease inhibitor is Tosyl-L-lysyl-chloromethane hydrochloride (TLCK) being present in said defined culture medium in a concentration that inhibits degradation of a WNT3 polypeptide, wherein the culture medium is capable of maintaining human pluripotent stem cells in a pluripotent state when cultured in a suspension culture devoid of substrate adherence, for at least 2 passages.
Dependencies are inferred from the claim text.