System and method for the production of biomolecules such as viral vaccines

A system for purifying target biomolecules, the system comprising:

• an upstream process compartment comprising
• a perfusion bioreactor;
• a growth medium tank adapted to provide a growth medium to the bioreactor;
• an intermediate vessel;
• a concentrator adapted to separate the target biomolecule for concentration in the intermediate vessel for harvest;
• and
• a plurality of conduits comprising a first conduit for transferring the liquid and the target biomolecules between the bioreactor and the intermediate vessel, a second conduit for transferring the liquid and the target biomolecules between the intermediate vessel and the concentrator, a third conduit adapted for recycling liquid exiting the bioreactor back to the growth medium tank for redelivery into the bioreactor, a retentate conduit for collecting a liquid output from said concentrator and recirculating said liquid output to an input of said intermediate vessel positioned between said concentrator and said bioreactor, and a harvest conduit adapted to harvest concentrated target biomolecules from the intermediate vessel;
• and
• a clarification compartment adapted to receive the concentrated target biomolecules;
• wherein said compartments are located in one or more cabinets, isolators, or containment enclosures.

A system for producing concentrated target biomolecules, the system comprising:

• a bioreactor including a chamber for receiving a liquid, the bioreactor adapted for producing target biomolecules;
• an intermediate vessel;
• a concentrator adapted to separate the target biomolecule for concentration in the intermediate vessel for harvest;
• and
• a plurality of conduits comprising
• at least one first conduit for transferring the liquid and target biomolecules between the chamber of the bioreactor and the intermediate vessel,
• at least one second conduit for transferring the liquid and target biomolecules between the intermediate vessel and the concentrator,
• at least one third conduit adapted for recycling liquid exiting the bioreactor to a growth medium tank for redelivery into the bioreactor, and
• at least one retentate conduit for collecting a retentate from said concentrator and facilitating recirculation of the retentate to an input of said intermediate vessel positioned between said concentrator and said bioreactor for collecting a concentrated target biomolecule in the intermediate vessel.