Cell culture system, method for using cell culture system, and cell culture unit

A cell culture system for culturing cells using a culture bag made of soft packaging material, including:

• a culture unit having a cutout portion or a transparent mounting portion on which a bag housing component having a pressing member that presses the culture bag is mounted;
• and
• the culture bag placed on the bag housing component, a medium vessel supplying a medium to the culture bag, a waste liquid vessel into which the medium is removed from the culture bag, first pumping means provided in a first tubular member connecting the culture bag and the medium vessel, and second pumping means provided in a second tubular member connecting the culture bag and the waste liquid vessel, on the culture unit,
• wherein a distance from a bottom surface of the culture unit to a culture surface of the culture bag is shorter than 20 mm,
• wherein the pressing member presses the culture bag filled with the medium to uniformize a liquid thickness of the medium in the culture bag such that a surface of the culture unit, an upper surface and a lower surface of the pressing member, and an upper surface and the culture surface of the culture bag become approximately parallel to one another.