Method for producing oligonucleotides

Abstract

Provided herein is a method for producing oligonucleotides, that curbs the amount of nucleoside phosphoramidites remaining in a reaction container in a synthesis step, and that reduces the usage amount of nucleoside phosphoramidites. This method for producing oligonucleotides includes: (a) detaching a protecting group from a protected nucleoside that is directly or indirectly supported on a carrier and that has the protecting group bonded to a hydroxyl group, a thiol group, or an amino group located at the 3′-position or the 5′-position; (b) in the presence of an activating reagent, forming a bond between a nucleoside phosphoramidite and the hydroxyl group, the thiol group, or the amino group located at the 3′-position or the 5′-position of the nucleoside from which the protecting group had been detached and which is directly or indirectly supported on the carrier; (c) sulfurizing or oxidizing the bond formed in the step (b); and (d) capping an unbound hydroxyl group, thiol group, or amino group located at the 3′-position or the 5′-position in the nucleoside directly or indirectly supported on the carrier.

Dependencies are inferred from the claim text.