Biochemical test module and manufacturing method thereof

A biochemical test module, comprising:

• a first reaction piece, comprising:
• a first substrate, having a first surface and a second surface opposite to the first surface;
• a first separator, located on the first surface and having a first flow channel;
• and
• a first cover, covering the first separator and having a first hole in connection with the first flow channel, wherein the first reaction piece has a perforated structure penetrating the first cover, the first separator and the first substrate;
• and
• a second reaction piece, comprising:
• a second substrate, having a third surface and a fourth surface opposite to the third surface, wherein the third surface faces the second surface;
• a second separator, located on the fourth surface and having a second flow channel;
• and
• a second cover, covering the second separator and having second hole, wherein the second hole is in connection with the second flow channel.

A method for manufacturing a biochemical test module, comprising:

• providing a first reaction piece, which comprises:
• a first substrate, having a first surface and a second surface opposite to the first surface;
• a first separator, located on the first surface and having a first flow channel exposing a first portion of the first substrate;
• and
• a first cover, covering the first separator and having a first hole in connection with the first flow channel;
• forming a perforated structure penetrating the first cover, the first separator and the first substrate;
• aligning a second sampling port of a second reaction piece with a first sampling port of the first reaction piece, wherein the second reaction piece comprises:
• a second substrate;
• a second separator, located on the second substrate and having a second flow channel exposing a second portion of the second substrate;
• a second cover, having a third surface covering the second separator and a fourth surface opposite to the third surface, and having a second hole in connection with the second flow channel, wherein the second flow channel is in connection with the perforated structure via the second hole;
• and
• attaching the second reaction piece to the first reaction piece.