Simplified method of preparing cells for patient administration
AssigneeSeattle Children’s Hospital d/b/a Seattle Children’s Research Institute
InventorAdam Beebe
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Abstract
The current disclosure describes simplified methods of preparing cells for patient infusion where the simplified methods result utilize less steps than conventional methods, decreasing required manipulation steps and reducing the time between beginning of cell manipulation for administration and ultimate administration to a patient. Methods of cryopreserving, thawing, and diluting cells and kits for practicing the methods are also provided herein.
Claims98
A method of preparing a therapeutic cell product for administration to a subject comprising
- transferring a volume of therapeutic cells into a first sterile receptacle to create a cell-filled sterile receptacle;
- transferring a specified volume of diluent into a second sterile receptacle to create a diluent-filled sterile receptacle;
- connecting the cell-filled sterile receptacle to the diluent-filled sterile receptacle with a fluid dispensing connector;
- and
- mixing the volume of cells with the volume of diluent thereby preparing the therapeutic cell product for administration to the subject.
A method for formulation of therapeutic cells for administration to a subject comprising thawing cryopreserved cells to create thawed cells;
- transferring a volume of thawed cells into a first syringe to create a cell-filled syringe;
- transferring a specified volume of diluent into a second syringe to create a diluent-filled syringe;
- connecting the cell-filled syringe to the diluent-filled syringe with a fluid dispensing connector;
- mixing the volume of cells with the volume of diluent to create a total mixed volume;
- and transferring the total mixed volume into either the cell-filled syringe or the diluent-filled syringe.
A kit for dilution of cell product comprising syringes, needles, fluid dispensing connector, and diluent.
Dependencies are inferred from the claim text.