Interaction of fibroblasts and immune cells for activation and uses thereof
AssigneeSpinalCyte, LLC
InventorsPete O'HEERON, Thomas ICHIM
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Abstract
The present disclosure is directed to systems, methods, and compositions for functional interaction of fibroblasts with one or more types of immune cells such that the interaction results in modification to the fibroblasts, the one or more types of immune cells, or both. In some embodiments, one or more certain agents are also utilized during the interaction or in lieu of one of the types of cells. In specific embodiments, cells to be used in cellular transplantation therapy are modified to have reduced immunogenicity.
Claims243
An in vitro method of producing activated fibroblasts or activated immune cells or activated immune cell derivatives, comprising the step(s) of:
- (a) exposing fibroblasts to immune cells under conditions such that one or more agents from the immune cells activates the fibroblasts to become activated fibroblasts and such that one or more antigens from the fibroblasts triggers production of one or more cytokines from the immune cells to produce activated immune cells;
- and/or
- (b) exposing fibroblasts to one or more growth factors and/or one or more cytokines under conditions such that the one or more growth factors and/or one or more cytokines activates the fibroblasts to become activated fibroblasts.
A method of reducing the immunogenicity of a cell population, wherein the population is subjected to a composition comprising IFN-gamma and optionally one or more additional agent(s) and/or condition(s).
A method of treating an autoimmune or inflammatory condition in an individual comprising administering to the individual a composition comprising a therapeutically effective amount of cells subjected to IFN-gamma and optionally one or more additional agent(s) and/or condition(s).
A method for generating a population of regulatory T cells for cell therapy, comprising the step of exposing a composition comprising activated fibroblast cells, and optionally one or more additional agent(s), to CD25+ T regulatory cells.
A method of treating liver failure in an individual, comprising the step of administering an effective amount of a cell population to the individual, wherein said cell population is a fibroblast cell population that has been exposed to immune cells;
- mesenchymal stem cells, CD34+ cells;
- very small embryonic like stem cells;
- Sertoli cells;
- or a mixture thereof.
A method of treating virally-induced hepatic failure in an individual, comprising administering to the individual an effective amount of a population of fibroblast cells that have been preconditioned with one or more stress-inducing stimuli.
A method of generating angiogenic macrophages comprising the steps of:
- a) obtaining a monocyte and/or monocyte progenitor cell;
- and b) contacting said monocyte and/or monocytic progenitor cell with fibroblast cells under conditions to endow said monocyte and/or monocytic progenitor cell with ability to stimulate angiogenesis.
A method of treating an ischemic condition in an individual, comprising the step of administering to the individual a composition comprising a therapeutically effective amount of cells subjected to IFN-gamma or PGE-2, and optionally one or more additional agent(s) and/or condition(s).
A method of inducing dendritic cell maturation comprising the steps of exposing immature dendritic cells to stressed fibroblast cells.
A method of enhancing one or more activities of a CD8 T cell, comprising the step of exposing stressed fibroblasts to immune cells or CD8 T cells produced or obtained from the immune cells.
A method of treating Graft Versus Host Disease in an individual, comprising the step of providing to the individual an effective amount of fibroblasts to the individual with a cell graft, tissue graft, or organ graft, and/or during or after said graft(s).
A method of cardiac regeneration in an individual in need thereof, comprising the step of administering to the individual an effective amount of a co-culture of fibroblasts and monocytes.
Dependencies are inferred from the claim text.