Pharmaceutical compositions for preventing or treating liver diseases with fibrosis
AssigneesShibuya Corporation, Yamaguchi University
InventorsNaoki YAMAMOTO, Taro TAKAMI, Toshihiko MATSUMOTO, Daiki KAWAMOTO
Life of this patent
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Abstract
It is an object of the present invention to provide a drug for preventing or treating a liver disease associated with fibrosis, the drug containing microRNAs as an active ingredient. A pharmaceutical composition for preventing or treating a liver disease associated with fibrosis, the pharmaceutical composition containing at least one kind of microRNAs selected from miR 1237-5p, miR 204-3p, miR 7977, miR 6089, and miR 5787 as an active ingredient, is produced.
Claims12
A pharmaceutical composition for preventing or treating a liver disease associated with fibrosis, the pharmaceutical composition comprising at least one kind of microRNAs selected from miR 1237-5p, miR 204-3p, miR 7977, miR 6089, and miR 5787, or precursors thereof as an active ingredient.
An extracellular vesicle derived from cells, obtained by introducing a polynucleotide encoding at least one kind of microRNAs selected from extraneous miR 1237-5p, miR 204-3p, miR 7977, miR 6089, and miR 5787, or precursors thereof into cells, and allowing the microRNAs or precursors thereof to be expressed therein,
- the extracellular vesicle comprising at least one kind of microRNAs selected from miR 1237-5p, miR 204-3p, miR 7977, miR 6089, and miR 5787, or precursors thereof.
A culture product obtained by introducing a polynucleotide encoding at least one kind of microRNAs selected from extraneous miR 1237-5p, miR 204-3p, miR 7977, miR 6089, and miR 5787, or precursors thereof into cells, allowing the microRNAs or precursors thereof to be expressed in the cells, and culturing the cells.
A method for producing extracellular vesicles for suppressing liver fibrosis, the extracellular vesicles containing at least one kind of microRNAs selected from extraneous miR 1237-5p, miR 204-3p, miR 7977, miR 6089, and miR 5787, or precursors thereof, the method comprising the following steps A to C:
- step A of introducing a polynucleotide encoding at least one kind of microRNAs selected from extraneous miR 1237-5p, miR 204-3p, miR 7977, miR 6089, and miR 5787, or precursors thereof into mesenchymal stem cells;
- step B of culturing the mesenchymal stem cells obtained in step A to obtain a culture product;
- and
- step C of harvesting extracellular vesicles from the culture product.
Dependencies are inferred from the claim text.