Varin genes
AssigneePhylos Bioscience, Inc.
InventorsErica Bakker, Alisha Holloway, Annie Poczynek
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Abstract
Provided herein is the identification of the causal gene KR associated with THCV, THCVA, CBDV, CBDVA, CBGV, CBGVA, CBCV, or CBCVA production in Cannabis plants. Also included are isolated cells, nucleic acids, transgenic Cannabis plants; methods of editing, and methods of using marker assisted selection to select and establish plant lines having modified THCV, THCVA, CBDV, CBDVA, CBGV, CBGVA, CBCV, or CBCVA activity.
Claims28
A transgenic Cannabis plant whose genome comprises a ketoacyl-acyl carrier protein reductase (KR) gene comprising one or more amino acid substitutions as compared to an endogenous KR gene and wherein the Cannabis plant comprises modified varin levels.
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An isolated nucleic acid sequence encoding one or more amino acid substitutions of at least a portion of an endogenous Cannabis KR gene.
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A method for selecting one or more Cannabis plants having modified varin levels, the method comprising:
- i) obtaining nucleic acids from a sample plant(s) or their germplasm;
- (ii) detecting one or more markers that indicate a modified varin level phenotype,
- (iii) indicating the modified varin level phenotype, and
- (iv) selecting the one or more plants indicating the modified varin level phenotype.
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Dependencies are inferred from the claim text.