Real-time chemical screening method using liver organoids fluorescently labeled with cytochrome p450 1a1 enzyme

Abstract

The present disclosure relates to a real-time chemical screening method using hepatic organoids fluorescently labeled with cytochrome P450 1A1 enzyme. The method for screening an AHR modulator or a hazardous chemical using CYP1A1 fluorescently labelled human pluripotent stem cell line-derived hepatic organoids according to the present disclosure enables the detection of substances that induce CYP1A1 regulation with higher sensitivity than when using the conventional human pluripotent stem cell line-derived hepatocytes by using hepatic organoids that can mimic the human body, and thus can be usefully utilized for early screening of new toxic or carcinogenic compounds while replacing animal testing.

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