Adhesive cell tissue gels
AssigneeNational Cheng Kung University
InventorLynn L.H. Huang
Life of this patent
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Abstract
A cell tissue gel, comprising one or more matrix molecules cross-linked with a cross-linking agent, and a quenching agent bound to a reactive group of the cross-linking agent, wherein the quenching agent contains a moiety that is capable of reacting with the reactive group of the cross-linking agent and the one or more matrix molecules contain one or more functional groups that are capable of cross-linking with the reactive group, the amount of the reactive group of the cross-linking agent being equal to or less than a total amount including the amount of the one or more functional groups and the amount of the moiety.
Claims5
A method of making a non-toxic adhesive cell tissue gel, comprising:
- mixing one or more matrix molecules with a quenching agent or a cross-linking agent to obtain a mixture;
- and
- adding a cross-linking agent or a quenching agent to the mixture;
- whereby the non-toxic adhesive cell tissue gel is formed;
- wherein the quenching agent contains a moiety that is capable of reacting with the reactive group of the cross-linking agent and the one or more matrix molecules contain one or more functional groups that are capable of cross-linking with the reactive group, the moiety being in an amount sufficient to react with any reactive group not cross-linked to the one or more functional groups and to reduce the cytotoxicity of the cell tissue gel cross-linked with the cross-linking agent.
A non-toxic adhesive cell tissue gel kit, comprising:
- one or more matrix molecules;
- a quenching agent;
- and
- a cross-linking agent, wherein the cross-linking agent is selected from a group consisting of an epoxide, a polyepoxy compound, a dialdehyde, an imidoester, a N-hydroxysuccinimide ester, a carbodiimide, genipin, a riboflavin, a flavonoid, a maleimide, a haloacetyl, a pyridyl disulfide, a hydrazide, an azide, a diazirine, a 6-maleimidohexanoic acid active ester, disuccinimidyl suberate, bis(sulfosuccinimidyl)suberate, and sulfosuccinimidyl-4-(N-maleimidomethyl) cyclohexane-1-carboxylate,
- wherein the quenching agent contains a moiety that is capable of reacting with the reactive group of the cross-linking agent and the one or more matrix molecules contain one or more functional groups that are capable of cross-linking with the reactive group, the moiety being in an amount sufficient to react with any reactive group not cross-linked to the one or more functional groups and to reduce the cytotoxicity of the cell tissue gel cross-linked with the cross-linking agent.
Dependencies are inferred from the claim text.