Biosynthetic hemostat and uses thereof

A biosynthetic hemostat comprising:

• a biocompatible flexible nanoparticle that includes a shell that defines an outer surface of the nanoparticle and a core, which is loaded with thrombin, and a plurality of von Willebrand factor-binding peptides (VBPs) and collagen-binding peptides (CBPs) that are linked to the shell and extend from the outer surface, wherein the nanoparticle is configured to adhere to a vascular surface, vascular disease site and/or vascular injury site with exposed von Willebrand factor (vWF) and collagen, shield the loaded thrombin in circulation to avoid rapid inhibition or systemic thrombotic risk, and release the loaded thrombin at the vascular surface, vascular disease site and/or vascular injury site by diffusion from the nanoparticle and/or enzyme triggered degradation of the nanoparticle.