Purification of phycobiliproteins
AssigneeFermentalg
InventorsOlivier CAGNAC, Axel ATHANE, Julien DEMOL
Life of this patent
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Abstract
The present invention relates to a novel process for purifying phycobiliproteins, in particular acid-pH-resistant phycobiliproteins, the resulting phycobiliproteins, and the uses thereof.
Claims18
Process for purifying acid-pH-resistant phycobiliproteins from a crude extract of acid-pH-resistant phycobiliproteins from cells of phycobiliprotein-producing microorganisms, the process comprising the steps of:
- a) adjusting the pH of the crude extract of acid-pH-resistant phycobiliproteins to a pH below 6 so as to precipitate organic matter other than acid-pH-resistant phycobiliproteins,
- b) recovering the supernatant comprising acid-pH-resistant phycobiliproteins and
- c) isolating acid-pH-resistant phycobiliproteins from the supernatant.
Acid-pH-resistant phycocyanin comprising a mixture of c-phycocyanin (C-PC) and allophycocyanin (APC), characterized in that the C-PC/APC molar ratio is at least 5.
(canceled)
Phycocyanin comprising a mixture of allophycocyanin (APC) and c-phycocyanin (C-PC) with a C-PC/APC molar ratio of less than 5.
Dependencies are inferred from the claim text.