Deregulated dhs and tyra enzymes of grasses enable efficient production of both tyrosine and phenylalanine
OwnerHiroshi Maeda, Jorge El Azazinventors
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Abstract
The present invention provides engineered cells and plants that express deregulated aromatic amino acid synthesis pathway enzymes from grasses. Methods for increasing the production of aromatic amino acids and their derivatives in cells and plants by engineering them to express these enzymes and methods for producing aromatic amino acids or derivatives thereof and/or sequestering carbon dioxide by growing the plants are also provided.
Claims20
A cell engineered to express or overexpress an enzyme selected from:
- a) a 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase 1b (DHS1b) enzyme comprising SEQ ID NO:
- 3 (BdDHS1b), SEQ ID NO:
- 19 (OsDHS1b), SEQ ID NO:
- 27 (SvDHS1b) or a DHS1b enzyme having at least 95% identity to one of SEQ ID NOs:
- 3, 19 or 27;
- or
- b) a noncanonical TyrA enzyme comprising SEQ ID NO:
- 37 (BdTyrAnc), SEQ ID NO:
- 43 (SbTyrAnc) or a TyrA enzyme having at least 95% identity to SEQ ID NO:
- 37 or 43;
- wherein (i) the cell is engineered to express an enzyme that is not native to the cell, or (ii) the cell is engineered to overexpress an enzyme that is native to the cell as compared to a control cell.
A method for increasing production of one or more aromatic amino acid or derivative thereof in a cell, the method comprising engineering the cell to express or overexpress an enzyme selected from:
- c) a DHS1b enzyme comprising SEQ ID NO:
- 3 (BdDHS1b), SEQ ID NO:
- 19 (OsDHS1b), SEQ ID NO:
- 27 (SvDHS1b) or a DHS1b enzyme having at least 95% identity to one of SEQ ID NOs:
- 3, 19 or 27;
- or
- d) a noncanonical TyrA enzyme comprising SEQ ID NO:
- 37 (BdTyrAnc), SEQ ID NO:
- 43 (SbTyrAnc), or a TyrA enzyme having at least 95% identity to SEQ ID NO:
- 37 or 43;
- wherein (i) the cell is engineered to express an enzyme that is not native to the cell, or (ii) the cell is engineered to overexpress an enzyme that is native to the cell as compared to a control cell.
Dependencies are inferred from the claim text.