Chimeric enzyme and method for producing terminal alkenes
AssigneeCnpem- Centro Nacional De Pesquisa Em Energia E Materiais
InventorsLeticia Maria ZANPHORLIN MURAKAMI, Wesley CARDOSO GENEROSO, Gabriela FELIX PERSINOTI
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Abstract
The present invention refers to a chimeric enzyme constructed for the production of terminal alkenes from fatty acids, so that the cofactor necessary for the reaction is produced in situ from industrial waste, favoring the circular economy. Furthermore, the present invention deals with the process of producing terminal alkenes using said chimeric enzyme. This invention belongs to the field of industrial biotechnology and finds application in the biofuels and renewable chemicals industry.
Claims15
Chimeric enzyme for the production of terminal alkenes characterized by comprising the decarboxylase enzyme SEQ ID NO:
- 1 joined in its terminal portion to the beginning of the alditol oxidase enzyme SEQ ID NO:
- 2 by means of a linker sequence.
Process for the production of terminal alkenes characterized by comprising the steps of:
- a) Contacting chimeric enzyme with a fatty acid in the presence of glycerol, forming a reaction medium;
- b) Keeping the reaction medium under heating and stirring for a period of time, and
- c) Collecting the product.
Dependencies are inferred from the claim text.