Genetically engineered bacterium for the production of 3-hydroxybutyrate
AssigneeAlliance for Sustainable Energy, LLC
InventorsJonathan LO, Jonathan Richard HUMPHREYS, Wei XIONG, Katherine Jenyan CHOU
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Abstract
Disclosed herein are novel methods and compositions of matter to produce 3HB in acetogens by using a(S)-3-hydroxybutyryl-CoA dehydrogenase, Hbd2, responsible for endogenous 3HB production. In conjunction with the heterologous thiolase atoB and CoA transferase ctfAB, hbd2 overexpression improves yields of 3HB on both sugar and syngas (CO/H2/CO2), outperforming previously disclosed pathways.
Claims20
A non-naturally occurring Clostridium sp. comprising a heterologous ctfAB gene and a hbd2 gene.
A method for making (S)-3-hydroxybutyrate comprising providing a carbon source to a solution comprising a non-naturally occurring Clostridium sp. comprising a heterologous ctfAB gene and a hbd2 gene.
Dependencies are inferred from the claim text.