Systems and devices for signal corrections in sequencing

A system comprising:

• one or more processors;
• and
• one or more storage devices storing instructions that, when executed by the one or more processors, cause the system to:
• measure intensities utilizing light sensors directed to a sample surface that includes a plurality of clusters of nucleic acids;
• generate, utilizing a progressive decay function, cycle-dependent coefficients for intensity decay at different cycles of a sequencing run;
• adjust a subset of the measured intensities using the cycle-dependent coefficients;
• and
• determine an intensity of a tag signal for a cluster of nucleic acids of the plurality of clusters based on the adjusted subset of the measured intensities.

A non-transitory computer readable medium storing instructions that, when executed by one or more processors, cause a system to:

• measure intensities utilizing light sensors directed to a sample surface that includes a plurality of clusters of nucleic acids;
• generate, utilizing a progressive decay function, cycle-dependent coefficients for intensity decay at different cycles of a sequencing run;
• adjust a subset of the measured intensities using the cycle-dependent coefficients;
• and
• determine an intensity of a tag signal for a cluster of nucleic acids of the plurality of clusters based on the adjusted subset of the measured intensities.

A computer-implemented method comprising:

• measuring intensities utilizing light sensors directed to a sample surface that includes a plurality of clusters of nucleic acids;
• generating, utilizing a progressive decay function, cycle-dependent coefficients for intensity decay at different cycles of a sequencing run;
• adjusting a subset of the measured intensities using the cycle-dependent coefficients;
• and
• determining an intensity of a tag signal for a cluster of nucleic acids of the plurality of clusters based on the adjusted subset of the measured intensities.